Andrei V Khokhlatchev

Charlottesville, VA

Higher Education

University of Virginia Health System since Jan 2003
Assistant Professor
University of Virginia 2010 - 2012
Senior Scientist
Harvard Medical School Jan 1998 - Jan 2003
Postdoctoral Fellow and Research Fellow in Medicine
UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER Sep 1997 - Feb 1998
Postdoctoral Fellow
University of Texas Southwestern Medical Center at Dallas Sep 1993 - Aug 1997
Graduate Student

Harvard Medical School
MOSCOW STATE UNIVERSITY
Master of Science, Biochemistry UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER
Doctor of Philosophy, Cell

Signal Transduction
Animal Models
Cell Biology
Protein Expression
Cancer Research
Transfection
Immunoprecipitation
Immunofluorescence
Molecular Cloning
Tissue Culture
Western Blotting
Fluorescence Microscopy
Cell Culture
Protein Purification
Microscopy
In Situ Hybridization
Pcr
Immunology
Molecular Biology
Cell Signaling
Protein Chemistry
Mutagenesis
Immunoassays
Assay Development
Dna

English
Russian

University of Virginia

2010 to 2000
Senior Scientist
University of Virginia
Charlottesville, VA
2003 to 2010
Assistant Professor
Massachusetts General Hospital and Harvard Medical School
Boston, MA
1998 to 2003
Postdoctoral Fellow
University of Texas Southwestern Medical Center
Dallas, TX
1993 to 1998
Graduate Student and Postdoctoral Fellow
University of Texas Southwestern Medical Center
Dallas, TX
1991 to 1993
Research Associate
Shemyakin Institute of Bioorganic Chemistry USSR Academy of Sciences

1984 to 1991
Research Trainee, Junior Research Fellow

University of Texas Southwestern Medical Center
Dallas, TX
1997
PhD
Moscow State University
1984
MS in Biochemistry

Molecular Biology: cloning, PCR, RT-PCR, mutagenesis, RNA isolation and reverse transcription, sequence analysis, design of expression constructs, shRNA vectors and oligonucleotides, design and assembly of TALEN and CRISPR/Cas9 encoding vectors. Cell Culture: plasmid and siRNA transfection, virus production and infection, apoptotic assays Proteins: characterization by SDS-PAGE, Western blotting, ELISA, immunoprecipitation, in vitro kinase and protease assays. Protein expression in E. coli and mammalian cells. Protein purification including IMAC, FLAG, myc epitopes and GST affinity chromatography, SEC, IEC, analytical and preparative low-pressure column, FPLC and HPLC. Microscopy: light, immunofluorescent and confocal fluorescent Microinjections of DNA and proteins in cytoplasm and nuclei of cultured cells Animal models: tumor growth in athymic mice, rabbit immunization and serum production, development of polyclonal and monoclonal antibodies. Zebrafish: microinjections in embryos, in situ hybridization, colony maintenance, use of TALEN and CRISP-R genome editing tools for fish mutagenesis. Computer Skills: MS Word, PowerPoint, Excel. Adobe Photoshop. PDRAW32 and Seqweb (analysis of DNA sequences). IPLab (Computer-based image capture and processing). Internet database use: ExPASy Bioinformatics resource portal, NCBI, Medline, BLAST sequence analysis and others.