US Patent:
20020031776, Mar 14, 2002
Inventors:
Richard Tullis - Encinadas CA, US
Jerome Streifel - San Diego CA, US
International Classification:
C12Q001/68
C12P019/34
Abstract:
Methods and markers for identifying and detecting nucleic acids using a linear amplification scheme are provided. The methods use chain extending enzymes, such as telomerases, to label probes hybridized to target nucleic acid products, including amplification products, to render them readily detectable. The methods may be performed as homogeneous or heterogeneous reactions. The methods provided herein can be used with any known method involving detection of a hybridized probe.