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Keith Patrick Bjornson

from Fremont, CA
Age ~56
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Also known as:
Keith P Bjornson, Keith Patrick Dr Bjornson, Keith N
Phone and address:
5268 Keeler Ct, Fremont, CA 94536
Related to:
Wyatt S Clark, 37Richard Ramey, 46Terry Bjornson, 68Debra Clark, 62Gerald L Clark, 54
Connected to:
Austin Bjornson, 32Denise L Bjornson, 63Erik R Bjornson, 41Graig Bjornson, 55Jill I Bjornson, 77Kerri J Bjornson, 49Kris M Bjornson, 79Matthildur K Bjornson, 101Max Bjornson, 38Nadezhda V Bjornson, 63

Keith Bjornson Phones & Addresses

  • 5268 Keeler Ct, Fremont, CA 94536
  • Union City, CA
  • 821 205Th St SE, Bothell, WA 98012
  • Newark, CA
  • 1833 Newbridge Ave, San Mateo, CA 94401
  • Durham, NC
  • Birmingham, AL
  • Roseburg, OR
  • Alameda, CA
  • Richmond Heights, MO

Publications

Us Patents

Methods And Compositions For Nucleic Acid Sample Preparation

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US Patent:
8236499, Aug 7, 2012
Filed:
Mar 27, 2009
Appl. No.:
12/383855
Inventors:
Pranav Patel - Fremont CA, US
Keith Bjornson - Newark CA, US
Kevin Travers - Santa Clara CA, US
Cheryl Heiner - San Mateo CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00
US Classification:
435 61, 435 611, 435 911, 435 9152, 536 231, 536 243, 536 2433, 536 253
Abstract:
Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.

Compositions And Methods For Use In Analytical Reactions

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US Patent:
8252911, Aug 28, 2012
Filed:
Feb 12, 2009
Appl. No.:
12/370472
Inventors:
Keith Bjornson - Newark CA, US
Arek Bibillo - Cupertino CA, US
Lubomir Sebo - Redwood City CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C07H 21/02
C12Q 1/68
C07H 21/04
US Classification:
536 221, 435 612, 536 2433
Abstract:
Compositions, methods, substrates and systems for use in analysis of single molecule reactions and particularly single molecule nucleic acid sequence analysis. Compositions that include non-reactive, distinguishable or undetectable competitive substrates for the reaction system of interest are provided, as well as their use in systems and substrates for such applications, such compounds typically preferably polyphosphate chains or analogous structures.

Generation Of Modified Polymerases For Improved Accuracy In Single Molecule Sequencing

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US Patent:
8257954, Sep 4, 2012
Filed:
Mar 30, 2009
Appl. No.:
12/384112
Inventors:
Sonya Clark - Oakland CA, US
Arek Bibillo - Cupertino CA, US
Paul Peluso - San Carlos CA, US
Fred Christians - Los Altos Hills CA, US
Molly He - Palo Alto CA, US
Insil Park - Fremont CA, US
Harold Lee - Sunnyvale CA, US
Keith Bjornson - Newark CA, US
Lei Jia - Palo Alto CA, US
Robin Emig - Belmont CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12N 9/12
C12P 19/34
US Classification:
435194, 435183, 435 911
Abstract:
Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

Generation Of Modified Polymerases For Improved Accuracy In Single Molecule Sequencing

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US Patent:
8420366, Apr 16, 2013
Filed:
Sep 30, 2010
Appl. No.:
12/924701
Inventors:
Sonya Clark - Oakland CA, US
Arek Bibillo - Cupertino CA, US
Paul Peluso - San Carlos CA, US
Fred Christians - Los Altos Hills CA, US
Molly He - Palo Alto CA, US
Insil Park - Fremont CA, US
Harold Lee - Sunnyvale CA, US
Keith Bjornson - Newark CA, US
Lei Jia - Palo Alto CA, US
Robin Emig - Belmont CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12N 9/12
C12P 19/34
US Classification:
435194, 435183, 435 911
Abstract:
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Immobilized Nucleic Acid Complexes For Sequence Analysis

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US Patent:
8481264, Jul 9, 2013
Filed:
Sep 18, 2009
Appl. No.:
12/562690
Inventors:
Keith Bjornson - Newark CA, US
Arek Bibillo - Cupertino CA, US
Fred Christians - Los Altos Hills CA, US
Kevin Travers - Santa Clara CA, US
Robin Emig - Belmont CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68
C12P 19/34
C07H 21/04
US Classification:
435 612, 435 912, 536 2433
Abstract:
Provided are methods for sequencing a nucleic acid that include fixing a template to a surface through a template localizing moiety and sequencing the nucleic acid with a sequencing enzyme, e. g. a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid fixed to a surface via a template localizing moiety, and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Compositions for sequencing reactions are provided.

Method For Sequencing Using Branching Fraction Of Incorporatable Nucleotides

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US Patent:
8530164, Sep 10, 2013
Filed:
Sep 4, 2009
Appl. No.:
12/584481
Inventors:
Pranav Patel - Fremont CA, US
Jonas Korlach - Newark CA, US
Arkadiusz Bibillo - Cupertino CA, US
Keith Bjornson - Newark CA, US
Jeremiah Hanes - Redwood City CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68
C12P 19/34
C07H 21/04
US Classification:
435 612, 435 912, 536 231
Abstract:
Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.

Methods And Compositions For Nucleic Acid Sample Preparation

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US Patent:
8535886, Sep 17, 2013
Filed:
Jun 12, 2012
Appl. No.:
13/494729
Inventors:
Pranav Patel - Fremont CA, US
Keith Bjornson - Newark CA, US
Kevin Travers - Santa Clara CA, US
Cheryl Heiner - San Mateo CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00
US Classification:
435 61, 435 611, 435 612, 435 911, 435 912, 536 231, 536 243, 536 2433, 536 253
Abstract:
Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.

Two Slow-Step Polymerase Enzyme Systems And Methods

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US Patent:
20090286245, Nov 19, 2009
Filed:
Mar 30, 2009
Appl. No.:
12/414191
Inventors:
Keith Bjornson - Newark CA, US
Harold Lee - Sunnyvale CA, US
Jonas Korlach - Newark CA, US
Stephen Turner - Menlo Park CA, US
Arek Bibillo - Cupertino CA, US
Fred Christians - Los Altos Hills CA, US
Robin Emig - Belmont CA, US
Molly He - Palo Alto CA, US
Insil Park - Fremont CA, US
Lei Jia - Palo Alto CA, US
Andrei Fedorov - San Mateo CA, US
John Lyle - Redwood Shores CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.
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Keith Patrick Bjornson from Fremont, CA, age ~56 Get Report