US Patent:
20210040490, Feb 11, 2021
Inventors:
- Ames IA, US
Mingfeng Cao - Ames IA, US
Miguel Suastegui - Ames IA, US
Meirong Gao - Ames IA, US
International Classification:
C12N 15/81
C12N 9/10
C12N 9/02
C12P 5/00
C12N 9/88
C12N 15/10
C12P 7/44
C12P 7/42
Abstract:
The present disclosure includes methods and components for production of valuable industrial compounds in yeast. In an embodiment, the present invention provides a nucleic acid construct with increased stability for gene expression or gene editing comprising: a nucleic acid sequence encoding one or more of SEQ ID NO: 1-8 (CENs 1-8); and one or more regulatory elements functional in a yeast cell. In an embodiment of the present invention the nucleic acid constructs are vectors, preferably episomal vectors. High expression promoters, as well as methods for increasing production of compounds such as aromatics are disclosed.