Inventors:
Neela Swaminathan - Madison WI
Richard K. Wilkosz - New Berlin WI
Assignee:
Molecular Biology Resources, Inc. - Milwaukee WI
International Classification:
C12N 912
C12N 1554
C12P 1934
Abstract:
The present invention is directed to an isolated and purified DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Bacillus stearothermophilus. More particularly, the invention is directed to a DNA encoding an approximately 66,000 dalton DNA polymerase that lacks 273 amino acids from the N-terminus of the approximately 96,000 dalton B. stearothermophilus DNA polymerase I, and to the protein encoded thereby which has been designated the B. stearothermophilus DNA polymerase I exo- fragment. The enzyme fragments are useful in DNA sequencing, cDNA preparations, thermophilic Strand Displacement Amplification and other molecular biology applications.