Inventors:
Richard L. Gehant - South San Francisco CA, US
International Classification:
C07K 1/14
C07K 16/40
C07K 16/22
US Classification:
5303892, 530350, 530412, 5303871, 5303893, 530427, 530417
Abstract:
The invention includes a process for extracting a target protein from cells that includes lowering the pH of a whole cell solution to form an acidic solution, disrupting the cells to release the protein into the acidic solution, and separating the cellular debris from the released protein to obtain a protein product enriched in the heterologous target protein. The invention also includes addition of a solubility enhancer.