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Alexis Sauer-Budge

from Lincoln, MA
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Also known as:
Alexis F Sauer, Alexis Sauer Budge
Connected to:
Alvin W Sauer, 105Brendon J Sauer, 30Brittany S Sauer, 35Carlton F Sauer, 101Chet D Sauer, 75Deanna Sauer, 40Debora L Sauer, 67Delores J Sauer, 76Dorrie J Sauer, 78Elba L Sauer, 77

Alexis Sauer-Budge Phones & Addresses

  • Lincoln, MA
  • Brighton, MA
  • Somerville, MA
  • Stanford, CA
  • Superior, CO

Work

Company: Fraunhofer usa - cmi Jul 2007 Position: Senior research scientist

Education

Degree: Ph.D. School / High School: Harvard University 1997 to 2002 Specialities: Biophysics

Skills

Assay Development • Medical Devices • Microbiology • Infectious Diseases • R&D • Biotechnology • Biochemistry • Lifesciences • Cancer • Life Sciences • Biomems • Chemistry • Research and Development • Cell Culture • Laboratory • Molecular Biology • Commercialization • Technology Transfer • Hardware Diagnostics • Protein Chemistry • High Throughput Screening

Languages

English

Industries

Research

Resumes

Resumes

Alexis Sauer-Budge Photo 1

Managing Scientist

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Location:
San Diego, CA
Industry:
Research
Work:
Fraunhofer USA - CMI since Jul 2007
Senior Research Scientist
Education:
Harvard University 1997 - 2002
Ph.D., Biophysics Stanford University 1993 - 1997
B.S./M.S., Chemistry
Skills:
Assay Development
Medical Devices
Microbiology
Infectious Diseases
R&D
Biotechnology
Biochemistry
Lifesciences
Cancer
Life Sciences
Biomems
Chemistry
Research and Development
Cell Culture
Laboratory
Molecular Biology
Commercialization
Technology Transfer
Hardware Diagnostics
Protein Chemistry
High Throughput Screening
Languages:
English

Publications

Us Patents

Methods And Apparatus For Detecting Viruses Using An Acoustic Device

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US Patent:
7632638, Dec 15, 2009
Filed:
May 2, 2006
Appl. No.:
11/416997
Inventors:
Alexis F. Sauer-Budge - Lincoln MA, US
Brett P. Masters - Watertown MA, US
Michael Miller - Hollis NH, US
Mark Lundstrom - Boston MA, US
Assignee:
BioScale, Inc. - Cambridge MA
International Classification:
C12Q 1/00
C12Q 1/70
G01N 33/543
G01N 33/553
B32B 27/00
B32B 27/12
US Classification:
435 5, 435 4, 435 71, 4352872, 4352873, 436523, 436526, 436149, 422 681, 422 81, 422 8213, 422100, 422104
Abstract:
Methods for detecting viruses are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for the virus, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Methods And Apparatus For Assay Measurements

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US Patent:
8227261, Jul 24, 2012
Filed:
May 2, 2006
Appl. No.:
11/416999
Inventors:
Brett P. Masters - Watertown MA, US
Michael F. Miller - Hollis NH, US
Alexis F. Sauer-Budge - Lincoln MA, US
Assignee:
BioScale, Inc. - Lexington MA
International Classification:
G01N 33/553
US Classification:
436526, 436523, 436525, 436168, 435 71, 435 794, 4352872, 4352873, 422 681, 422 81, 73 2401, 73580, 73599, 73863
Abstract:
Methods and apparatuses for performing assays involving binding material elements with a plurality of bonds over a substantial area of a surface of a resonant device establishing a normalized exposure. The methods and apparatuses also involve controlling an external influence applied to the material elements over a first period of time and measuring a signal during a second period of time that is indicative of the change in the amount of material elements bound to the surface relative to the normalized exposure. In some cases, the measured signals are integrated with respect to time to determine the time averaged amount of material elements bound to the surface.

Methods And Apparatus For Detecting Bacteria Using An Acoustic Device

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US Patent:
20070037231, Feb 15, 2007
Filed:
May 2, 2006
Appl. No.:
11/416998
Inventors:
Alexis Sauer-Budge - Lincoln MA, US
Eric Fitch - Arlington MA, US
Brett Masters - Watertown MA, US
Michael Miller - Hollis NH, US
Mark Lundstrom - Boston MA, US
Assignee:
BioScale, Inc. - Cambridge MA
International Classification:
G01N 33/554
G01N 33/569
US Classification:
435007320, 977902000
Abstract:
Methods for detecting bacteria are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for the bacteria, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Isolation Of Rna

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US Patent:
20110313145, Dec 22, 2011
Filed:
Jun 10, 2011
Appl. No.:
13/157929
Inventors:
Andre Sharon - Newton MA, US
Anirban Chatterjee - Malden MA, US
Paul Mirsky - Jamaica Plain MA, US
Alexis Sauer-Budge - Lincoln MA, US
Assignee:
FRAUNHOFER USA, INC. - Plymouth MI
TRUSTEES OF BOSTON UNIVERSITY - Boston MA
International Classification:
C07H 1/08
B01D 35/00
C12M 1/24
US Classification:
536 2541, 4353061, 422535
Abstract:
Disclosed herein are methods for purification of RNA from a sample. The RNA can be total RNA or mRNA. The method involves preparing the sample in a solution of lysis buffer and depositing into a first end of a lysis straw such that the sample solution flows through the matrix of the lysis straw and is eluted from the opposite end of the lysis straw, and depositing the eluted material into a first end of a solid phase extraction (SPE) straw, such that the deposited solution flows through the matrix of the SPE straw towards the opposite end of the SPE straw, and eluting the RNA from the SPE straw by depositing a solution of elution buffer, into the first end of the SPE straw, such that the deposited solution flows through the matrix of the SPE straw and is eluted from the opposite end of the SPE straw, wherein purified RNA from the sample is present in the eluate of the SPE straw. When the RNA is total RNA, and the sample is a cell sample, and step b) requires adding a precipitating solution to the eluted material from step a), and depositing the solution into the first end of the SPE straw, wherein the straw comprises silica microspheres, such that the deposited solution flows through the matrix of the SPE straw toward the opposite end of the SPE straw. When the RNA is mRNA and step b) further comprises depositing the solution into the first end of the solid phase extraction (SPE) straw, wherein the straw comprises oligo-dT. Pressure (e.g., at least 10 psi pressure) and heat (e.g., about 60 C.) can be applied to the samples, and/or eluates and/or straws. Examples of lysis buffers, loading buffers and elution buffers are provided. Also disclosed are the specific straws and porous polymer monolith matrix components.

Reservoir-Buffered Mixers And Remote Valve Switching For Microfluidic Devices

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US Patent:
20120077260, Mar 29, 2012
Filed:
Mar 30, 2010
Appl. No.:
13/262570
Inventors:
Andre Sharon - Newton MA, US
David A. Chargin - Somerville MA, US
Paul Mirsky - Jamaica Plain MA, US
Alexis Sauer-Budge - Lincoln MA, US
Assignee:
Fraunhofer USA, Inc. - plymouth MI
Trustees of Boston University - Boston MA
International Classification:
C12M 1/40
G01N 21/75
B01L 3/00
US Classification:
4352872, 422502, 4352891, 422 8205
Abstract:
The present invention relates generally to the control of fluid flow rate and direction on a microfluidic device. In particular, the present invention provides an integrated valveless microfluidic device, where directional fluid control is controlled using off-chip remote valve switching and fluid flow rate changes are controlled using on-chip flow-rate changing fluid reservoirs. The present invention provides methods and systems for directional fluid control and control of fluid flow rate in an integrated microfluidic device which enables processes with different flow rates to be performed on one device without the need of on-chip valves.

Disposable Separator/Concentrator Device And Method Of Use

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US Patent:
20120115705, May 10, 2012
Filed:
Apr 30, 2010
Appl. No.:
13/266980
Inventors:
Andre Sharon - Newton MA, US
Alexis Sauer-Budge - Lincoln MA, US
Aaron Size - Waltham MA, US
Holger Wirz - Medford MA, US
Assignee:
Fraunhofer USA, Inc. - Plymouth MI
Trustees of Boston University - Boston MA
International Classification:
B04B 1/16
B01D 21/26
B04B 15/00
US Classification:
494 4, 494 56, 494 5, 494 10, 494 12, 494 26, 494 37
Abstract:
The present invention relates to methods, devices and systems for separation and concentration of particles from liquid and fluid samples. In some embodiments, the separation/concentration is achieved by sequential centrifugation steps. In particular, one aspect of the invention relates to a separation/concentration device which comprises at least a first chamber () and a second chamber () connected by a first valve (), whereby operation of the first valve controls the material transfer from the first chamber to the second chamber. In some embodiments, valve operation can be manually, semi-manually or automatically. Other aspects of the invention relate to single- or multi-chambered separation/concentrator devices, and methods and systems for use. Other aspects of the invention relate to devices for operation of the valves, e.g., semi-manual actuation devices, and automatic inertial activation devices and mechanical actuation devices present in purpose-built centrifuges.

Method And Device For Rapid Detection Of Bacterial Antibiotic Resistance/Susceptibility

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US Patent:
20120122831, May 17, 2012
Filed:
Oct 28, 2011
Appl. No.:
13/283892
Inventors:
Alexis Sauer-Budge - Lincoln MA, US
Andre Sharon - Newton MA, US
Maxim Kalashnikov - Chestnut Hill MA, US
Holger Wirz - Medford MA, US
Assignee:
FRAUNHOFER USA, INC. - Plymouth MI
TRUSTEES OF BOSTON UNIVERSITY - Boston MA
International Classification:
A61K 31/431
C12M 1/00
A61P 31/04
C12Q 1/18
US Classification:
514196, 435 32, 4352879
Abstract:
Described herein is a method and a device for expediting delivery of an agent to a damaged bacterial cell. In one embodiment, the methods and devices are useful for screening candidate antibiotics. In another embodiment, the methods and devices described herein are used to determine susceptibility of bacteria to an antibiotic. The methods also provide a method for determining an appropriate antibiotic to treat an individual having a bacterial infection.

Characterization Of Individual Polymer Molecules Based On Monomer-Interface Interactions

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US Patent:
20130270115, Oct 17, 2013
Filed:
Mar 28, 2013
Appl. No.:
13/852606
Inventors:
Alexis Sauer-Budge - Brighton MA, US
Jene A. Golovchenko - Lexington MA, US
Amit Meller - Newton MA, US
Eric Brandin - Portsmouth NH, US
Daniel Branton - Lexington MA, US
International Classification:
G01N 27/447
US Classification:
204456
Abstract:
The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.
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Alexis Sauer-Budge from Lincoln, MA Get Report