US Patent:
20130157870, Jun 20, 2013
Inventors:
Dmitry Pushkarev - Stanford CA, US
Stephen R. Quake - Stanford CA, US
Ayelet Voskoboynik - Aptos CA, US
Michael Kertesz - Menlo Park CA, US
Assignee:
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY - Palo Alto CA
International Classification:
C12Q 1/68
US Classification:
506 2, 506 36, 435 915, 435 9153, 435 9152, 435 611, 435 612
Abstract:
The invention generally relates to methods for obtaining a sequence, such as a consensus sequence or a haplotype sequence. In certain embodiments, methods of the invention involve determining an amount of amplifiable nucleic acid present in a sample, partitioning the nucleic acid based upon results of the determining step such that each partitioned portion includes, on average, a subset of unique sequences, sequencing the nucleic acid to obtain sequence reads, and assembling a consensus sequence from the reads.
