US Patent:
20210140947, May 13, 2021
Inventors:
- West Lafayette IN, US
Aimable Ngendahimana - West Lafayette IN, US
Christopher D. Kulczar - Bremen IN, US
Kelsey E. Lubin - Lafayette IN, US
Monika Lavan - Lafayette IN, US
Assignee:
Purdue Research Foundation - West Lafayette IN
International Classification:
G01N 33/50
C12N 5/079
C12N 5/071
Abstract:
The present disclosure generally relates to a process to prepare a cell culture system that mimics the structure of blood brain barrier (BBB) and are useful to study the functions thereof. In particular, the present invention relates to a direct-contact coculture and triculture systems prepared by plating BMECs on a pre-formed lawn of coculture of astrocytes and pericytes on the apical surface of a culture-chamber to achieve a truly direct contact triculture model for BBB. The cell culture systems disclosed herein are also useful for studying the functions of the blood brain barrier and predicting the efficacy and potential toxicity of a drug candidate.