Dan A Pankowsky

6828157, Dec 7, 2004

May 3, 2000

09/563564

Dan A. Pankowsky - Nashville TN, 37205

G01N 33553

436176, 435 2, 435 721, 435 723, 435 724, 435 75, 435 794, 435961, 436518, 436523, 436524, 436536, 436527, 436528, 436538, 436540, 436824, 436174, 436175, 436177, 436178

A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.

7351589, Apr 1, 2008

Nov 12, 2004

10/988039

Dan A. Pankowsky - Nashville TN, US

G01N 1/18

436177, 436524, 436526, 436528, 436533, 436534, 436538, 436540, 436 10, 436164, 436165, 436172, 435 2, 435 72, 435 794, 435 4051, 422101

A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.

7364906, Apr 29, 2008

Dec 18, 2003

10/739832

Dan A. Pankowsky - Nashville TN, US

G01N 31/00

436 10, 436524, 436526, 436528, 436533, 436534, 436538, 436540, 436164, 436165, 436172, 436177, 435 2, 435 72, 435 794, 435 4051, 422101

A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody-coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are contacted simultaneously or sequentially with a suspension of cells and bind the cells they are adapted to bind to form bead-cell complexes. Cells may bind to one or more microspheres. The bead-cell complexes are then separated from the suspension The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A method of quantitating a specific cell type is provided. A kit and apparatus for performing the method are also provided.

7537907, May 26, 2009

Feb 6, 2008

12/027010

Dan A. Pankowsky - Nashville TN, US

G01N 33/574

435 723, 435 2, 435 72, 435 792, 435 4051, 4352872, 436 64, 436164, 436165, 436172, 436177, 436524, 436526, 436528, 436533, 436534, 436538, 436540, 436 10, 422101

A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.

D663856, Jul 17, 2012

Apr 7, 2010

29/359166

Joel Lieblein - Sudbury MA, US
Ronald Lasser - Andover MA, US
Dan Pankowsky - Franklin TN, US
Jill Gershon - Franklin TN, US
Daniel Jones - Concord MA, US

2401

D24216

20010041343, Nov 15, 2001

Jul 20, 2001

09/909677

Dan Pankowsky - Nashville TN, US

C12Q001/68
G01N033/567

435/006000, 435/007210

A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody-coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are contacted simultaneously or sequentially with a suspension of cells and bind the cells they are adapted to bind to form bead-cell complexes. Cells may bind to one or more microspheres. The bead-cell complexes are then separated from the suspension The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A method of quantitating a specific cell type is provided. A kit and apparatus for performing the method are also provided.

20110300618, Dec 8, 2011

Apr 7, 2011

13/081593

Joel Lieblein - Sudbury MA, US
Ronald Lasser - Andover MA, US
Dan Pankowsky - Franklin TN, US
Jill Gershon - Franklin TN, US
Daniel Jones - Concord MA, US

C12M 1/34

4352871

A single-use device that produces a readily interpretable, visual presentation of biomaterial, enabling single parameter and multi-parameter characterizing of said biomaterial by means of visual inspection, particularly for immunophenotyping. In one embodiment, the device uses antibody coated microspheres which are adapted to bind to specific types of cells. An arrangement of uniquely molded components, which may be plastic or other suitable material, are assembled so as to provide a sequential procedure capable of incubating a suspension comprised of antibody coated microspheres, biomaterial, and liquid suspension agents. Following incubation, the invention further removes liquids from said suspension, and provides for rinsing the incubated retentate and for removing said rinsing liquids from the incubated retentate. The invention further provides for affixing the incubated retentate onto a standard glass microscope slide to enable phenotyping of incubated biomaterial (e.g., cells) by means of visual inspection or other analyses that can be performed from a glass slide.