Inventors:
- Cambridge MA, US
Hesham Azizgolshani - Belmont MA, US
Brian P. Cain - Cambridge MA, US
Brett C. Isenberg - Newton MA, US
Joseph L. Charest - Jamaica Plain MA, US
Else M. Vedula - Stoneham MA, US
Ashley L. Gard - Dorchester MA, US
Ryan S. Maloney - Cambridge MA, US
Jeffrey T. Borenstein - West Roxbury MA, US
Rebeccah Lulu - Cambridge MA, US
Assignee:
The Charles Stark Draper Laboratory Inc. - Cambridge MA
International Classification:
C12M 3/06
C12M 1/32
C12M 1/00
C12M 1/12
Abstract:
The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.