Ilke Akartuna

20180304222, Oct 25, 2018

May 29, 2018

15/991600

- Cambridge MA, US
- Vilnius, LT
Ilke Akartuna - Cambridge MA, US
Linas Mazutis - Vilnius, LT
Marc W. Kirschner - Newton MA, US

B01J 19/00
B01F 13/00

The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

20180071705, Mar 15, 2018

Oct 3, 2017

15/723490

- Cambridge MA, US
- Vilnius LA, US
Ilke Akartuna - Cambridge MA, US
Linas Mazutis - Vilnius LA, US
Marc W. Kirschner - Newton MA, US

B01J 19/00
B01F 13/00

The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

20160129444, May 12, 2016

Jun 12, 2014

14/896218

- Cambridge MA, US
Thomas E. Kodger - Cambridge MA, US
Donald Aubrecht - Somerville MA, US
Ilke Akartuna - Cambridge MA, US

President and Fellows of Harvard College - Cambridge MA

B01L 3/00

The present invention generally relates to microfiuidics, and, in particular, to systems and methods for coalescing or fusing droplets. In certain aspects, two or more droplets within a microfluidic channel are brought together and caused to coalesce without using electric fields or charges. For example, in certain embodiments, droplets stabilized with a surfactant may be disrupted, e.g., by exposing the droplets to a solvent able to alter the surfactant, which may partially destabilize the droplets and allow them to coalesce. In some instances, the droplets may also be physically disrupted to facilitate coalesce. In addition, in some cases, the positions of one or more droplets may be controlled within a channel using a groove in a wall of the channel. For example, a droplet may at least partially enter the groove such that the position of the droplet is at least partially controlled by the groove.

20150298091, Oct 22, 2015

Jun 9, 2015

14/734903

- Cambridge MA, US
- Vilnius, LT
Ilke Akartuna - Cambridge MA, US
Linas Mazutis - Somerville MA, US
Marc W. Kirschner - Newton MA, US

B01J 19/00

The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.