Jainamma A Krotz

7270850, Sep 18, 2007

Mar 16, 2005

11/082325

Jainamma Krotz - San Diego CA, US
Daniel J. Smolko - Jamul CA, US
Howard R. Reese - Poway CA, US
Thomas J. Onofrey - San Marcos CA, US
Daguang Wang - San Diego CA, US
Theodore M. Winger - San Diego CA, US
John R. Havens - San Diego CA, US

Nanogen, Inc. - San Diego CA

C23C 16/00
B01D 29/00

42725528, 210490, 2105001, 21050035, 436172, 435 6, 4352872, 204400, 204403, 422 681, 422 50

The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array by treating the surface with a linker by vapor deposition and providing a polymerization solution that includes at least one monomer having a polymerizable moiety, a modified streptavidin, a surfactant or porogen, and a cross-linking agent. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker.

7597932, Oct 6, 2009

Sep 14, 2007

11/855374

Jainamma Krotz - San Diego CA, US
Daniel Smolko - Jamul CA, US
Howard R. Reese - Poway CA, US
Thomas J. Onofrey - San Marcos CA, US
Daguang Wang - San Diego CA, US
Theodore M. Winger - San Diego CA, US
John R. Havens - San Diego CA, US

Nanogen, Inc. - San Diego CA

C23C 16/00
B01D 29/00
C12Q 1/68
C12M 1/34

42725528, 435 6, 4352872, 204400, 20440301, 204450, 210490, 21050035, 2105001

The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array and providing a polymerization solution that includes a porogen. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker. The porogen is then removed from the permeation layer, thereby creating void spaces in the permeation layer.

20030146145, Aug 7, 2003

Dec 10, 2001

10/014895

Jainamma Krotz - San Diego CA, US
Daniel Smolko - Jamul CA, US
Howard Reese - Poway CA, US
Thomas Onofrey - San Marcos CA, US
Daguang Wang - Vista CA, US
Theodore Winger - San Diego CA, US
John Havens - Arlington MA, US

B01D063/08

210/243000, 210/490000, 210/500220, 210/321840, 210/500380, 422/101000, 216/056000

The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The permeation layers have a defined porous character, with mesopores in a size range between about 100 nanometers and about 1000 nanometers, and may also have micropores in the micrometer size range. The mesoporous synthetic hydrogel permeation layers demonstrate improved signal intensity and linearity characteristics as compared to nanoporous synthetic hydrogel permeation layers on active electronic matrix devices. In addition, the present invention also provides synthetic polymer hydrogel permeation layers which contain copolymerized attachment sites for nucleic acid probes or other biomolecules.

20060166285, Jul 27, 2006

Jan 26, 2005

11/044739

Jainamma Krotz - San Diego CA, US
David Wong - San Diego CA, US
Kenny Nguyen - San Diego CA, US

G01N 33/53
C12M 1/34

435007500, 435287200

An electronic device including at least one electrode on a substrate and a permeation layer overlaying the at least one electrode. The permeation layer comprises a polymer having a plurality of negatively-charged moieties. The negatively charged moieties may be carboxylates. In one embodiment, the negatively charged moieties are carboxylates from acrylic acids in the permeation layer. The amount of acrylic acid may be less than about 5 mol %. The permeation layer may also contain streptavidin and/or a surfactant. Methods of using the electronic device are also disclosed in which at least one of the selectively addressable electrodes are biased to at least partially neutralize the negatively charged moieties in the overlying permeation layer. Charged entities can then bind to the permeation layer over the biased electrode.