US Patent:
20170306291, Oct 26, 2017
Inventors:
- Oakland CA, US
Yilin HU - Irvine CA, US
Johannes REBELEIN - Irvine CA, US
International Classification:
C12N 1/20
C12P 5/02
C07K 14/21
C12M 1/24
C12F 3/04
C12Q 1/04
C12P 5/02
C12P 3/00
Abstract:
In alternative embodiments, provided are genetically or recombinantly engineered nitrogen-fixing, nitrogenase expressing bacteria capable of enzymatically synthesizing hydrocarbons, and methods for making and using them. In alternative embodiments, provided are genetically or recombinantly engineered nitrogen-fixing, nitrogenase expressing bacteria including nitrogen-fixing diazotrophs such as nitrogen-fixing bacteria of the family or the genus for the whole cell synthesis of hydrocarbons and carbon-carbon bonds. In alternative embodiments, nitrogen-fixing, nitrogenase-expressing bacteria used to practice the invention are genetically or recombinantly engineered to express an exogenous nitrogenase express more endogenous nitrogenase or have increased nitrogenase, activity. In alternative embodiments, nitrogen-fixing, nitrogenase-expressing bacteria used to practice the invention are genetically or recombinantly engineered to lack or have decreased molybdenum transporter activity. In alternative embodiments, provided are culture systems, fermenters and bioreactors using nitrogen-fixing, nitrogenase-expressing bacteria for enzymatically synthesizing hydrocarbons.