Rini M Saxena

20030104367, Jun 5, 2003

Dec 3, 2001

09/683221

Carsten Rosenow - Mountain View CA, US
Rini Saxena - Mountain View CA, US

Affymetrix, Inc. - Santa Clara CA

C12Q001/68
C12P019/34
C12M001/34

435/006000, 435/091200, 435/287200

Methods are provided for detecting the sense and antisense transcripts and for determining template strand of a genomic DNA. Exemplary methods include reverse transcribing transcripts without second strand synthesis. The resulting single stranded DNA is labeled and detected using nucleic acid probe arrays. In a particularly embodiment, actinomycin is used to inhibit the synthesis of second strand cDNA during reverse transcription.

20030118989, Jun 26, 2003

Dec 21, 2001

09/683405

Carsten Rosenow - Redwood City CA, US
Rini Saxena - Mountain View CA, US

C12Q001/68
C12N001/08

435/006000, 435/270000

In one embodiment, methods are provided for differential isolation of cellular contents from different cells for further analysis. In one exemplary embodiment, the nucleic acids of bacteria are isolated from a mixture of bacteria and animal cells by lysing animal cells.

20040126798, Jul 1, 2004

Dec 5, 2003

10/728458

Carsten Rosenow - Redwood City CA, US
Rini Saxena - Mountain View CA, US

Affymetrix, INC. - Santa Clara CA

C12Q001/68

435/006000

Methods are provided for detecting the sense and antisense transcripts and for determining template strand of a genomic DNA. Exemplary methods include reverse transcribing transcripts without second strand synthesis. The resulting single stranded stranded DNA is labeled and detected using nucleic acid probe arrays. In a particularly preferred embodiment, actinomycin is used to inhibit the synthesis of second strand cDNA during reverse transcription.