Resumes
Resumes
Syed Zeyad Washington, DC
View pageWork:
AERAS Foundation
Rockville, MD
Dec 2006 to Dec 2014
Senior Research Specialist
The Henry M. Jackson Foundation
Rockville, MD
Jun 2000 to Dec 2006
Research Assistant
Rockville, MD
Dec 2006 to Dec 2014
Senior Research Specialist
The Henry M. Jackson Foundation
Rockville, MD
Jun 2000 to Dec 2006
Research Assistant
Education:
John's Hopkins University
Baltimore, MD
2007
M.S. in Biotechnology
University of Maryland
College Park, MD
2000
B. S. in Physiology/ Neurobiology
Baltimore, MD
2007
M.S. in Biotechnology
University of Maryland
College Park, MD
2000
B. S. in Physiology/ Neurobiology
Skills:
Research<br/> Microarray: Printing (SDDC-2 Microarray), Labeling (Genisphere 350RP) and Scanning (GenePix 4000A).<br/> Molecular Biology: PCR, Nested PCR, cDNA synthesis and RT-PCR, Quantitative PCR and Quantitative RT-PCR (Taqman), DNA and RNA extractions, Molecular cloning (Gel extractions, TA plasmid cloning, plasmid isolation, RE digestions, Ligations, BCG and E.coli Transformations), SDM( Site Directed Mutagenesis) and Sequencing. Familiar with Sequencher4.6 and Clone Manager Suite 7.<br/> Cell Biology: Cell line maintenance (BCG and mammalian cell lines), Apoptosis TUNEL Assays, Hemolysin Assay, ATPase Cell Viability Assay, Isolation of HIV-1 virus from PBMCs, HIV-1 MT-2 phenotyping assay, HIV-1 coreceptor usage in GHOST cell assay, p24 antigen capture assay, HIV-1 virus infectivity quantification (TCID50), transfections (Calcium phosphate and Fugene transfections), HIV-1 viral expansions, and. cell separation and purification using magnetic beads (Dynal and MACS systems).<br/> Biochemistry: Protein Expression, Column Protein Purification, Total Protein Extraction, BCA Protein Assay, SDS-PAGE (Comassie and Silver Staining) and Western Blot (AP and ECL).<br/> Flow Cytometry: BD FACSCalibur, Partec Cyfolw, Apoptosis TUNEL Assay Intracellular Cytokine Detection, Intracellular HIV-1 p24 detection (as an HIV-1 Neutralization endpoint), GFP detection, and cell surface staining.<br/><br/>Clinical<br... CD4 Phenotyping using BD Multiset (BD FACSCalibur) High Complexity Testing<br/> Chemistry using Automated Rouche COBAS Integra 400 Plus including electrolytes<br/> Hematology CBC/Diff using Automated Coulter Act5diff CP<br/> Urine Analysis using Bayer Health Care Clinitek Status Automated Urine Analyser and Multistix 10SG Strips.