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Wayne K Versaw

from College Station, TX
Age ~59
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Also known as:
Wayne Keith Versaw, Wayne E Versaw, Wayne I Versaw
Phone and address:
2911 Durango Ct, College Station, TX 77845
(979) 695-8532
Related to:
Marjorie M Versaw, 92Keith Versaw, 94Brooke A Versaw, 28Lori Versaw, 59
Connected to:
Donald L Versaw, 124Grant L Versaw, 56Karen S Versaw, 61Mackenzie L Versaw, 43Barbara M Verschatse, 81Joel D Versch, 39Megan N Verschage, 44Nicholas Verschaeve, 31Sharon A Versch, 80Alfred H Verschell, 97

Wayne Versaw Phones & Addresses

  • 2911 Durango Ct, College Station, TX 77845 (979) 695-8532
  • College Sta, TX
  • 1529 Red Oak Dr, Ardmore, OK 73401 (580) 226-3922
  • Madison, WI
  • Hastings, NE
  • Lincoln, NE

Publications

Us Patents

Process For Dephosphorylating Linear Polynucleotide Substrate With Prosphatase Form Aspergillus Niger

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US Patent:
54320640, Jul 11, 1995
Filed:
Oct 13, 1992
Appl. No.:
7/959543
Inventors:
John P. Markwell - Lincoln NE
Wayne K. Versaw - Madison WI
John C. Osterman - Waverly NE
Philip M. Kelley - Lincoln NE
Assignee:
Board of Regents of the University of Nebraska - Lincoln NE
International Classification:
C12N 916
C12N 114
C12N 100
C12P 1934
US Classification:
435 911
Abstract:
The present invention relates to the preparation of a novel heat-labile phosphatase enzyme from the filamentous fungus Aspergillus niger. This A. niger phosphatase enzyme has a native molecular weight of approximately 80,000 daltons, and is shown by polyacrylamide gel electrophoresis under denaturing conditions to be an alpha-2 dimer consisting of identical subunits of molecular weight of approximately 37,000 daltons each. The native intact enzyme molecule has an isoelectric point (pI) of 4. 6, and exhibits optimal functional activity under reaction conditions of neutral to slightly alkaline pH conditions (about pH 7. 0 to about pH 8. 5). This enzyme has two characteristics which make it valuable in molecular biology laboratory protocols. First, the enzyme is readily inactivated by mild heating conditions (50. degree. C. ); and second, the enzyme is highly specific for DNA as a substrate for the hydrolysis reaction; it does not hydrolyze adenosine triphosphate (ATP).

Heat-Labile Phosphatase

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US Patent:
51837528, Feb 2, 1993
Filed:
Oct 29, 1990
Appl. No.:
7/605539
Inventors:
John P. Markwell - Lincoln NE
Wayne K. Versaw - Madison WI
John C. Osterman - Waverly NE
Philip M. Kelley - Lincoln NE
Assignee:
Board of Regents of the University of Nebraska - Lincoln NE
International Classification:
C12N 916
C12N 100
C12P 1934
US Classification:
435196
Abstract:
The present invention relates to the preparation of a novel heat-labile phosphatase enzyme from the filamentous fungus Aspergillus niger. This A. Niger phosphatase enzyme has a native molecular weight of approximately 80,000 daltons, and is shown by polyacrylamide gel electrophoresis under denaturing conditions to be an alpha-2 dimer consisting of identical subunits of molecular weight of approximately 37,000 daltons each. The native intact enzyme molecule has an isoelectric point (pI) of 4. 6, and exhibits optimal functional activity under reaction conditions of neutral to slightly alkaline pH conditions (about pH 7. 0 to about pH 8. 5). This enzyme has two characteristics which make it valuable in molecular biology laboratory protocols. First, the enzyme is readily inactivated by mild heating conditions (50. degree. C. ); and second, the enzyme is highly specific for DNA as a substrate for the hydrolysis reaction; it does not hydrolyze adenosine triphosphate (ATP).
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Wayne K Versaw from College Station, TX, age ~59 Get Report